Your English writing platform
Discover LudwigSuggestions(1)
Exact(1)
It infers that, quantification of copies of this gene in extracted DNA samples from WSSV infected shrimp might be indicative of the number of virions and naked genome prior to assembly present in the infected or carrier host.
Similar(59)
For absolute quantification of transcript copy numbers, a standard curve of linearized plasmid (molecules/μl) was generated for each amplicon.
Absolute quantification of transcript copy number was achieved by generating calibration curve using plasmid DNA templates (listed above) as previously described [29,30].
For quantification of copy number of integrated viral vectors, genomic DNA (gDNA) was extracted (Sigma GenElute Genomic Mammalian DNA Mini prep kit) from eGFP+ FACS sorted cells cultured without MEF cells.
gDNA was used to generate standard curves for absolute quantification of copy number per reaction.
Genomic DNA (gDNA) was used to create standard curves for absolute quantification of copy number per reaction.
Digital PCR technology is very promising since it allows accurate quantification of copy numbers in contrast to other quantification methods such as real-time PCR.
For quantification of copy number of DENV-2 RNA, the standard curve was generated by 10-fold serial dilution of known copies of DENV-2 RNA.
Large genomic laboratories may want to use PRT as an easy method of replicating high-throughput data by an alternative method with accurate quantification of copy number.
The digest method allowed the presence or absence of the α-tryptase allele to be detected in an individual, but did not permit quantification of copy number.
Absolute quantification of copy number in the different species was subsequently done by interpolation of the threshold cycle number (Ct) against the corresponding standard curve.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com