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Quantification of calcium deposition was made by extracting Alizarin Red S staining with 10% cetylpyridinium chloride (CPC) and measuring the absorbance at 570 nm.
The production of a mineralized matrix is considered as the final process of osteoblast phenotype in differentiation by quantification of calcium deposition (Owen et al. 1990; Bancroft et al. 2002).
The osteogenic differentiation of hAMSCs on microcarriers was confirmed with increased mineral deposition (Alizarin red S staining and quantification of calcium content), ALP activity as well as gene expression of osteogenic markers (collagen type I and osteocalcin).
For the quantification of calcium levels, cells were washed with PBS and decalcified with 0.6 N HCl for 24 h, Calcium content was determined by measuring the concentrations of calcium in the HCl supernatant by atomic absorption spectroscopy.
Thus, quantification of calcium signaling was performed in the present study.
5) Quantification of calcium channel antibody staining lacks methodology and sample sizes.
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Quantification of coronary calcium was performed on a separate workstation with software for calcium scoring (Heartbeat-CS, EBW, Philips Medical Systems, Best, The Netherlands).
An experimentally feasible protocol, quantification of subcellular calcium alternans and restitution slope during cycle-length ramping alternans control, was designed and validated.
Quantification of the calcium deposition (alizarin red staining) supported these results demonstrating that over a 21 day period osteoblasts from both the uninfected and S. aureus SpA mutant stained for calcium deposition similarly (Fig 5C, P = NS), whereas S. aureus Newman and S. aureus complemented with pCU1Spa failed to do so (Fig 5C, P<0.01 and P<0.001, respectively).
For the quantification of coronary calcium 3D Leonardo application (Siemens, Munich, Germany) was used.
(D ) Quantification of mitochondrial calcium marks visualized with Rhod2-AM from isolated soleus muscle fibers from Ryr1 +/+ (black bars) and Ryr1 AG/+ (gray bars) mice.
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