Figure 4 summarizes quantification of blocking experiments.
Model Eqs. 1, 2, 3, 4, 5, 6, 7, 8 and 9 were applied in modelling the description and quantification of blocking mechanisms controlling the membrane processes.
After verifying the utility of MMIHC on a chip and examining repeatability and the possibility of quantification using cell blocks, we applied the platform to patient tumor tissues which are heterogeneous in terms of morphology, genetic alterations and histopathological features.
To bridge this gap, we established protocols employing specific antibody combinations for immunostaining, confocal imaging, three-dimensional reconstruction of approximately 100-μm-thick tissue blocks and quantification of key architectural features.
Flow cytometry, propidium iodide staining and ethanol fixation was used for the quantification of cell cycle progression, G2M block and the presence of apoptosis.
An increased number of autophagosomes can occur despite later steps of autophagy being blocked; the quantification of LC3-II [ 134] before and after the inhibition of autophagosome-lysosome fusion by using lysosomal inhibitors may indicate more accurately the autophagic flux [ 134].
This approach allows for the identification of certain regulatory features of the pathway and for the quantification of the behaviour of the reaction blocks towards to changes in the concentration of the fixed metabolite.
(E ) Quantification of GluA1 and TfR internalization and block of dynamin function.
The event-driven approach we use allows for the quantification of evolutionary conservation of any gene block.
(C ) Quantification of GluA1 and Tf-Alexa647 internalization and block of dynamin function.
c, d Quantification of [131I]-I2-PARPi [131I]-I2-PARPi [131I]-I2-PARPin-blocked, blocked, and healthy mice.
