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Absolute quantification of bacteria was performed as described in supplementary information.
The significance of 16S rRNA operon multiplicity to the quantification of bacteria is discussed.
For MoDCs invasion model and for quantification of bacteria within human cells each sample eCFU was normalized to the total cell count.
The experimental method allowed the accurate quantification of bacteria survival on photoactive surfaces and films under UVA and UV-free visible irradiation.
The rapid quantification of bacteria with flow-through chemiluminescence microarrays was established in our laboratories and is applied for bioaerosol analysis in this work.
Identification and quantification of bacteria affecting human life, directly causing diseases and indirectly contaminating natural ecosystems, are mostly carried out by expensive and time-consuming methods.
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Mantel-Cox tests were performed for survival curves and one-tailed Mann-Whitney paired tests were used to compare quantifications of bacteria, bacteriophages and cytokines.
The cells were washed twice with pre-warmed medium to remove extracellular bacteria before the quantification of intracellular bacteria was performed.
Quantification of expressed w.t.
Henry, S. et al. Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR.
In this work, a method based on quantitative PCR (qPCR) and targeting the gtf gene was developed for detection and quantification of these bacteria in cider.
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