Quantification of aggregate formation revealed that a huge amount of mAb consisted of aggregates.
Quantification of aggregate formation and LC3 dots were assessed as already previously described (16, 52).
Quantification of aggregate formation and LC3 dots was assessed as previously described (46).
Gel image quantification of aggregate proteins from conditioned media showed that some secreted protein species have extraction ratios of >100.
The quantification of aggregate-containing cells revealed that expression of the phosphomimetic NBR1 mutant counteracted the effect of inactive GSK3 on DES aggregation (Fig. 2E).
The quantification of aggregate intensities were carried out from SUM projected videos, and the intensity values were normalized to the surrounding cytoplasmic value.
Quantifications of aggregates number and size, or GFAP signal, were scored with ImageJ software from 256 μm × 256 μm images.
Since the quantification of these aggregates is an important aspect of this story, the authors should quantify aggregate formation with more vigor, using alternative procedures such as sucrose-density gradients and ultra-centrifugation to separate aggregates from other cellular debris.
It was shown that the two methods were in accordance for quantification of large aggregates, which were observed around slack tide when salinity decreased.
