A long time focus of research and development in our laboratory has been on the reliable and reproducible quantification of regional abnormalities in blood flow during physical and pharmacological stress.
In fact, quantification of chromosomal abnormalities induced by these agents is used for clinical diagnosis of FA.
For quantification of nuclear abnormalities ten fields were counted per slide and 3 slides were counted per experiment.
Agreement and kappa coefficients were >85% and >0.75, respectively, for interrater and intrarater variability in quantification of flow abnormalities during sepsis, in different subsets of microvascular architecture.
The aim of the process was to implement a systematic approach to the analysis of OPS derived microcirculatory flow imaging that would allow identification and quantification of microcirculatory abnormalities during critical illness.
We called together six collaborative centres involved in clinical microcirculation research in paediatric and adult intensive care units in the Netherlands to come to a consensus about quantification of microcirculatory abnormalities in direct observations obtained by means of OPS imaging.
Remarkably, quantification of microvascular abnormalities in sporadic and hereditary SVDs has shown that qualitatively the processes involved in arteriolar degeneration are largely similar in sporadic SVD compared with hereditary disorders such as cerebral autosomal arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
This promise can only be realised, however, when the obtained images are interpreted uniformly and quantification of microcirculatory flow abnormalities is reproducible.
Retinal imaging can facilitate the measurement and quantification of subtle variations and abnormalities in retinal vasculature.
Final manual correction of misclassification errors improved accuracy (similarity index range: 0.87 to 0.89) and facilitated objective quantification of white matter signal abnormalities.
