For quantification of IgG a standard curve of mouse IgG1 (BD) is tested in parallel.
Quantification was carried out using a LightCycler 480 Real-Time PCR System (Roche Diagnostics, Mannheim, Germany) by comparing the quantification cycle of each sample to the quantification cycle of a standard curve constructed by amplifying different known copy numbers of target gene.
For quantification of Se, a standard addition method was used to eliminate matrix effects of residual carbon and other matrix elements.
These data were confirmed and met the relative quantification requirements of a standard curve.
For limit of detection and limit of quantification a standard solution containing each of saccharin, phenacetin, paracetamol and methaqualone (1 mg/mL each) was diluted to give the different concentrations (0.05 μL/10 μL to 2 μL/10 μL).
The general concept is the quantification of a potential phosphorus standard via its protons using H qNMR, which is subsequently employed for the quantification of an analyte via its phosphorus using P qNMR.
To measure the accuracy of this quantification method, a standard curve was performed with different known proportions of alleles (known proportions of genomic DNA).
Surprisingly, few studies have used DNA quantification as a standard measure of the developmental cycle despite the suggestion a decade ago [9].
Pfscd and Pfactin copy numbers were determined by the absolute quantification method using a standard set of reactions of P. falciparum 106/1 genomic DNA of known copy number.
For quantification, a standard of pGEX 5×3 Hplasmid1 plasmid (Genscript, Piscataway, NJ, USA) and of a quantified BKPyV-positive urine sample were used.
