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However, if sufficiently large volume of interest was used, accuracies of quantification obtained from the point source and water cylinder were similar.
Marker frequency quantification, obtained from the standard curves shown in Figure 2. The sum of frequencies in each mix is shown on the right.
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This allowed us to compare emphysema quantifications obtained from data acquired with different parameters.
Copy number analyses were performed by using standard curves from artificial templates normalized for absolute quantifications obtained from miRNA-spiked.
In the upcoming open-source software framework CellSys, the quantifications obtained from TiQuant can directly be used as an input for the CellSys modelling engine.
Normalization allows comparison of emphysema quantification obtained with scanners from different manufacturers.
Chemical analysis of a liver biopsy is the "gold standard" for the measurement of LIC, but patients are resistant to this approach as it is invasive and, moreover, results vary widely: the coefficient of variation in the quantification obtained by liver biopsy is from 19% in a healthy liver up to 40% in the case of cirrhosis [4, 5, 6, 7].
The reproducibility of the quantification results obtained from the different samples demonstrates the robustness of the extraction and quantification steps (Table 3).
Numerous factors like peptide concentration and amino acid composition can influence the quality of spectra and hence the apparent quantification ratios obtained from iTRAQ experiments.
Since the initial analyses were based upon relative quantification data obtained from the non-targeted biochemical profiling technology, a targeted assay was developed to provide absolute quantitative results.
Quantification was obtained from at least three sections/animal and at least three animals.
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