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E Quantification number of number of whole cell and F aspect ratio.
(F and L ) Quantification; number of neuroblasts scored shown in bar.
Briefly, Relative quantification number (RQ) was obtained from the equation (1 + E 2∆∆CT where ∆∆CT represents ∆CT (Treatment -∆CT (ConTreatment -∆CT the PControlciency.
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DOI: http://dx.doi.org/10.7554/eLife.06156.016 10.7554/eLiFigure56.017 Figure 2 figure supplement 3. Quantification of number of EGFR and pEGFR molecules per endosome.
Slides were masked prior to each type of quantification (cell number and apoptosis as outlined below) to facilitate unbiased counting.
For parasitaemia quantification the number of parasites per 100 high-power microscopic fields was estimated and the parasite density was calculated from this value [52].
For quantification, the number of pHH3+ cells in the SVZ surrounding each lateral ventricle was counted.
The quantification cycle number (Cq) was determined using the absolute quantification/2nd derivative method [ 26].
(E ) Quantification of number of IHCs and OHCs indicates no difference between WT and Sema3f −/−.
(B ) Quantification of number of branch points in control and Msi2 overexpression mice.
Quantification of number of nuclei that stained positively for RNA DNA hybrids in srs2Δ and rdh54Δrad54Δ.
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