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All antisense RNA species are included in the quantification; note that RNAi reduces the level of the full-length antisense RNA but slightly increases some truncated species.
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The index was qualitative with a broad attempt at quantification noting whether less or more than half of the surface was affected.
Quantification: please note that the quantification of the segment gap phenotype was given in Figure 1E.
By quantifications, we noted that up to 7.84% and 18.9% of neuronals showed apoptotic death at 4 and 24 h by treatment with thapsigargin respectively.
In "Adverbs of Quantification" (1975a), Lewis notes several difficulties in translating sentences involving "usually", "frequently", "rarely" or related adverbs into first-order logic or some similar formal system.
For this reason, all subsequent analyses of acetylation stoichiometry are based on SILAC quantification (unless otherwise noted).
Note that quantification by the <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0012355.e044.PNG" class= inline-graphic"/> standard curve requires the entire dilution series, while estimates made by the other four quantification methods are based on single qPCR runs at each dilution.
Of note, the quantification of some types of expressed retroelements by using classical methods may prove ambiguous.
Note that quantification of the number of GFP molecules in RNPs undergoing motion was precluded by fluctuations in GFP fluorescence intensity.
We note that quantification of molecular glycerophospholipid species with two FA moieties is relatively straightforward as compared to molecular TAG species [ 27].
Note that quantification was not possible for all peptide variants1,2 and that the displayed abundances indicate the relative amounts among those variants that could be quantified.
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