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These modification and quantification methods are expected to be good technologies in manufacturing functional esophagus substitutes.
Current immobilized protein quantification methods are indirect, time-consuming, and/or inaccurate.
For the accomplishment of topical applications of LA appropriate drug quantification methods are essential.
Fast metabolite quantification methods are required for high throughput screening of microbial strains obtained by combinatorial or evolutionary engineering approaches.
It is therefore concluded that hardness-based or modulus-based damage quantification methods are intrinsically flawed and should not be used for the determination of a damage parameter.
To evaluate the quality of mixedness in particulate systems, either through experiments or with CFD simulations, proper quantification methods are necessary.
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In addition, multiple lines of experiments including different quantification methods were provided for consistent and mutually supportive results.
During this phase, initial hypotheses regarding the benefits and quantification methods were tested and data was collected.
These quantification methods were also investigated to assess their precision.
Therefore six different protein extraction protocols and seven protein quantification methods were tested and compared.
Quantification methods were optimized through three different real-time PCR chemistries, i.e. SYBR® Green I, Amplifluor™ and TaqMan®.
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