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A mean shift segmentation technique gave the best results, but for real-time constraints we simply implemented an image quantification method based on the HSL colour system.
Digital polymerase chain reaction (PCR) is an emerging absolute quantification method based on the limiting dilution principle and end-point PCR.
In order to characterizes and compensate for expression variations a quantification method based on Chromeo546-labled StrepTactinII was developed to quantify the number of FhuA Δ1-160 Δ1-160 outer E. colinmembrane (∼44000 of FhuA Δ1-160 per cell).
Cp values were converted to copy numbers using the absolute quantification method based on Cp values for the genomic DNA standards.
Our results demonstrate that the microtubule quantification method based on the shift in NM magnitude was robust enough to analyze multiple cell types under cytoskeleton destabilizing conditions.
We demonstrated that the significant variation in rDNA copy number per cell of O. cf. ovata in culture systems made it impossible to develop a reliable and accurate quantification method based only on a plasmid standard curve or only on a pool of DNA target from cultured samples.
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Ct and Cy0 quantification methods, based on cycle-threshold and the kinetic of amplification curves, respectively, were compared.
Most research studies have investigated AV-45 uptake using quantification methods based on target-to-cerebellum standard uptake values (SUVr) [ 5, 8, 16].
Consequently, we developed both JP2-clone- and non-JP2-clone-specific quantification methods based on primer specificity and the qPCR technique.
Although not specifically investigated here, the use of multiple proteases to increase protein sequence coverage should also improve quantification methods based on spectral counting.
Samples of myocardium were analyzed for AMP and ATP quantification by a method based on high performance liquid chromatography.
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