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Figure 1: DNA quantification level for each location.
Adding the concentrations needed for the standard curve, the H2B gene showed both detection level (DL) and quantification level (QL) at 5E-4 ng/μL.
The quantification level (QL), defined as the minimum of quantifiable DNA in the standard curve, was determined as the lowest DNA concentration with positive amplification and a CV lower than 0.3.
Our work considers SHM quantification level and in particular the evaluation of the severity of delamination-type damage in CFRP composite laminates.
In contrast, the autumn season was more variable (F = 140.377; df = 9; P = 0.000) with three significantly differentiated groups: Gaia presented the highest quantification level, Boadella Reservoir in an intermediate position and La Baells, La LLosa and Sant Ponç Reservoirs with the lowest and similar quantification levels (Fig. 1).
With the standard ADH1 as references, protein quantification level was exported in femtomol and nanogram.
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Lower quantification levels could be readily obtained by increasing counting time or incident flux and changing detectors.
These limits of quantification levels of the impurities were helpful for the research work in controlling the impurities at the accepted level during the optimization of the process.
Median quantification levels of all analyzed microbes were obtained from triplicate real-time PCR measurements.
In sharp contrast, GMO and cinnarizine were below quantification levels after 4 h.
For transcript quantification levels, RNA was extracted using TRIzol reagent (Invitrogen).
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