For quantification, labelled sections were analysed in five individual experiments by taking 25 53 micrographs including the detected regions of the parasitophorous vacuole membrane at a nominal magnification of 5000×.
Comparing to labeling-based quantification, label-free quantification avoids labeling steps, offers advantages related to the number of samples and the protein detection sensitivity, and has emerged as a high-throughput method in the investigation of scientific questions with clinical relevance over the years [ 30].
Except for the TMT quantification labels, carbamidomethylation of cysteine was set as a fixed modification, and oxidized methionine was set as a variable modification.
Furthermore, simultaneous quantification using labelling methods is only possible between few samples (Wang et al, 2008; Zhu et al, 2010).
Notably, quantification of labelling patterns of xylulose-5-P, dihydroxyacetone and glyceraldeyde phosphate, which are directly linked to methanol metabolism, could be measured by first time.
Right panel shows the quantification of labeled mtRNAs (n = 3).
Next to cell visualization, quantification of labeled cells implanted in cartilage defects by MRI could provide an exquisite opportunity to longitudinally map the role and contribution of implanted cells to cartilage repair.
Quantification of labeling intensity was normalized to labeling at the 4-hr time point after subtraction of background.
Although protein relative quantification using labeling strategies has been successfully used in many studies, these techniques are strongly limited by the number of samples that can be compared.
Quantification of labeled adiponectin uptake into tissues demonstrated uptake primarily into liver and kidney with no significant differences detected between vehicle and isosorbide dinitrate treatment for HMW adiponectin.
