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A biotin quantification kit was bought from Pierce Biotechnology (Rockford, IL, USA).
We also verified NAD+ formation using an NAD+/NADH quantification kit.
The protein extraction was quantified using BCA protein quantification kit (Dingguochangsheng Biotech Co. Ltd. Beijing, China).
Nucleic acid yields were checked using the Quant-iT PicoGreen dsDNA quantification kit (Invitrogen, Carlsbad, CA, USA).
In order to confirm the attachment of the biotin on the polymer, a biotin quantification kit was used.
After chemical cell disruption, the protein concentration of crude cell extract was determined using the Protein Quantification Kit BCA-Assay (Interchim, France) according to manufacturer´s instructions.
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Library quantification was performed using the KAPA Library Quantification Kit (KAPA Biosystems, Boston, MA).
The generated library was validated and quantified using Kapa Library Quantification kit (Kapabiosystem).
Quantitative-PCR library quantification was carried out using the Kapa Library Quantification Kit (Kapa Biosystems).
The quantification of all libraries was done using the Library Quantification Kit (Kapa Biosystems, Woburn, MA).
The global 5-mC and 5-hmC in DNA were quantified using the MethylFlash Methylated DNA Quantification Kit Catt P-10344, Epigentek) and the MethylFlash Hydroxymethylated DNA Quantification Kit Catt P-10366, Epigentek) according to the manufacturer's instructions.
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