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Quantification is gated on CD8+ T cells.
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As such, PR-5 is gated by an "or" gate.
What is gating?
As for the quantification of donor leukocyte's MHC-I expression, engrafted donor leukocytes were gated, and the fluorescence intensities (FIs, including mean, geographic mean, and median) of anti-H-2Kb PE were measured.
Lymphocytes, granulocytes and monocytes were gated.
First, CD3+/-cells were gated from the lymphocyte gate.
Cells were gated on CD45.
Monocytes were gated using CD14.
Measurement was gated with ECG.
We do not believe that FACS-gate quantification is consistent enough to say much more than the cells are present at relatively normal levels.
"Quantification is a technology of distance," as the historian of science Theodore Porter has pointed out.
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