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Quantitative results based on Ct are more accurate than results derived from end point determinations because Ct derived quantification is determined from measurements taken during the early exponential phase of PCR amplification before efficiency is affected by limiting reagents and product re-annealing.
PCR Array quantification is determined by CT numbers.
The absolute time-point of detection Td (quantification) is determined when the slope increase is > 30 mV for the first time at a tube scanner excitation intensity of 36%.
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The tissue was prepared and protein quantification was determined using the BCA protein assay.
Finally, the accuracy of the quantification was determined for the large uniform part of three different phantoms.
The limit of detection and quantification were determined for the three laboratory obtained fibres (MA, MMA and MA/MMA).
The limit of quantification was determined by identifying the next highest concentration on the standard curve with a signal-to-noise ratio of >10.
With regard to organochlorine pesticide analyses, the current practice for limits of detection and quantification was determined as part of the method development and validation.
The accuracy of the quantification was determined by comparing the measured activity with the known activity within the phantom measured at preparation.
Protein quantification was determined by BCA assay.
Absolute quantification was determined using a genomic DNA standard curve.
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