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However, the objectivity of the quantification increases by applying probabilistic assumptions.
The variability of the quantification increases notably when longer P2 cleaved transcripts are produced.
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Beyond traditional PCR, digital PCR for absolute quantification, increased robustness, and higher sensitivity is highly desirable.
These experiments are quite laborious, time-consuming and the number of manipulations required to achieve proper quantification increased the likelihood of introducing error.
It has been demonstrated here that the accuracy and efficiency of the Bayesian quantification algorithm increases if damage localization is carried out a-priori.
Also, the quantification accuracy increases by use of more than one reference gene [ 59].
Conducting absolute quantification greatly increased the resolution of the analysis, in addition to allowing the expression of any gene to be directly compared with that of any other gene, within and between multiple samples.
The amount of identification and quantification data increased dramatically during the recent years and resulted in the accumulation of "metadata", which means data about data.
Therefore, besides improving image quality, efforts need to be directed towards image quantification or increasing the accuracy of radionuclide activity measurement.
Quantification of increasing R. felis-infection was demonstrated at the whole flea level (counting Rf17kDa copy numbers) and verified again when assessing infection as a ratio of Rickettsia and flea genes.
It is possible that alternative modeling of the environmental risk factors or more precise exposure quantification would increase statistical power (e.g. analyzing intensity, duration or pack-years of smoking rather than as never/former/current).
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