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Beyond traditional PCR, digital PCR for absolute quantification, increased robustness, and higher sensitivity is highly desirable.
These experiments are quite laborious, time-consuming and the number of manipulations required to achieve proper quantification increased the likelihood of introducing error.
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The variability of the quantification increases notably when longer P2 cleaved transcripts are produced.
However, the objectivity of the quantification increases by applying probabilistic assumptions.
In qPCR assays performed on non-amplified GI-ME-N and LAN-5 cDNAs the quantification rates were 384 and 357 T-UCRs, respectively, whereas upon amplification the quantification rate increased on average of 12% (up to 438 and 423 T-UCRs in GI-ME-N and LAN-5, respectively).
The amount of identification and quantification data increased dramatically during the recent years and resulted in the accumulation of "metadata", which means data about data.
(E ) Quantification shows increased production of both TH+ and total neurons by selamectin in hiPSCs.
(C ) Quantification shows increased production of both TH+ and total neurons by selamectin in H9 hESCs.
However, the usefulness of fluctuations significantly expands with absolute quantification and increased numbers of proteins assayed per cell (multiplexing).
(B ) Quantification shows increased production of both TH+ and total neurons by selamectin (t -test, n = 4, p<0.001).
(D and E ) The quantification of increased dominant and subdominant MCC-reactive TCR pairs of CD4SP thymocytes or CD4+ peripheral T cells in B6.K or gp250 SC mice.
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