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Additionally, in the online version respondents were pointed out to the missing quantification in case of reporting 'yes' on the items.
However, an option for increasing quality of quantification in case of low amount of sample and missing replicated samples is to divide one sample into technical replicates and use more sensitive approaches like 2D fluorescent difference gel electrophoresis (2D-DIGE).
Sodium azide has no effect on the H NMR spectra of urine, whereas sodium fluoride causes a shift especially of the citrate resonances [ 2]; consequently sodium azide should be preferred over sodium fluoride for H NMR quantification in case the condition of the sample collection cannot be increased to higher standards.
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Serial dilution into buffy coat-derived MNC from a pool of ten healthy donors was used as standard curve (100%, 10%, 1%, 0.1%, and 0.01%) for quantification in cases and controls.
The diagnostic accuracy of FDG-PET/CT and hTERT mRNA quantification in cases of malignant tumors was estimated after stratification for the presence or absence of a malignant tumor, resulting in positive predictive values of FDG-PET/CT and hTERT mRNA quantification of 84.1% and 66.7% and negative predictive values of 95.3% and 67.8%, respectively.
As long as we restrict the domain of quantification in this case to include just the two bishops in question, this will yield the truth conditions that we are looking for.
Manual dilution was performed before quantification in the case of the highest concentrations.
For the 40-mer, typical assay conditions did not allow reliable quantification; in that case, concentrations of 50 μM 40-mer and 10 nM BoNT/A LC were used in the assay.
Therefore, the small RNAs in contaminated transcriptase might interfere with quantification in certain cases.
Since the normal human melanocytes did not grow well in the labeling media, heavy isotopes were incorporated into the Lau-Me275 cell line, and protein quantification in all cases was relative to this sample.
Plasma HIV RNA levels were below the limit of quantification in 1825 cases (71.3%).
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