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Before biofilm quantification, growth of wild type and mutant strains was assessed by measuring the absorbance of cultures in the wells at 600 nm with a spectrophotometer.
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For quantification of growth rates, distances between LAGs, i.e. growth zones were measured with Leica IM 50 Image Manager®, and annual growth rates per day were calculated [ 51] by dividing growth zones by the number of days per growth period and year.
Quantification of growth rates and response to therapy of orthotopic glioma models has been established using MRI [23], [24], [25].
(F ) Quantification of growth and competition phases.
(F ) Quantification of growth fitness for indicated mutant strains.
Further quantification showed growth of 1 200 000 000 cfu/L.
(A ) and (B ) Quantification of growth fitness for indicated strains (see Figure 2B, F ).
(A ) and (B ) Quantification of growth fitness for indicated mutant strains (see Figure 2F ).
(D ) Quantification of growth fitness for indicated mutant strains (see Figure 2F ).
(G ) Quantification of growth and retraction dynamics at branches using the tracer tool shows significant decrease in branch lengths in EGFRDN compared to control.
Nevertheless, both methods only allow the quantification of growth and viability on the culture level, giving an average over all cells, but not a quantification of the viable cells.
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