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The expression levels of ERa were categorized as follows (normalization against RNA of HKG RPS18) for consecutive quantification: group 0: no ERa standard mRNA detectable; group 1: ERa standard mRNA amount ≤0.81; group 2: ERa standard mRNA amount >0.81, due to the mean mRNA level within ERa standard positive samples being 0.81.
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Statistical significance comparing CD3+ and CD68+ infiltrated cells quantification between group I and group II was found (p < 0.001).
Quantification of group foraging - To quantify group foraging we took each bearing of each bat and determined which other bats' transmitter signal had been recorded within the same 30 second forward time window.
Out of 20 samples in each of the 4 categories, 10 samples were subjected to iTRAQ based quantification (iTRAQ group) and the rest of the samples were stored at −80°C for further use (validation group).
Set regular path queries (SRPQ) [50] extends regular path queries with quantification for group selection, to restrict the nodes in one set connected to the nodes of another.
The developed qPCR technique targeting the 16S-23S ITS region is both rapid and simple and is useful for selective quantification of group variations among sympatric Microcystis genotypes, such as in mixed cultures and the natural environment.
Quantification of group foraging - Our dataset from the canopy tower consisted of a total of 579 independent observations of bats (i.e., radio bearings that were 2 minutes apart).
(G ) Quantification across groups (n = 4) revealed that the level of cFos expression in DA neurons after exposure to sucrose is significantly lower in sweet blind Trpm5 −/− animals in which MCH neurons have been ablated vs controls.
For quantification of group-specific bacteria, we used the following primers: F, 5′-ATGGCTGTCGTCAGCTCGT-3′; R, 5′-CCTACTTCTTTTGCAACCCACTC-3′ (for Enterobacteriaceae) and F, 5′-CCCTTATTGTTAGTTGCCATCATT-3′; R, 5′-ACTCGTTGTACTTCCCATTGT-3′ (for Enterococcus spp .. Organoids from mouse colons were isolated and cultured in vitro as described (Sato et al., 2011).
Similarly to the experiments with DNA quantification, the diploid group was determined according to the peak of G0/G1 cells in the histograms.
Figure 5 shows a clear increase in apoptosis and a complete suppression of proliferation in the combination F16 IL2 plus temozolomide treatment group (quantification in Supplementary Figure 6).
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