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Normalised ratios of the target genes were, therefore, calculated using an efficiency-corrected relative quantification formula within the LightCycler Relative Quantification Software with either a lamina or stubble tissue sample of perennial ryegrass at the 3-leaf stage of regrowth as a calibrator.
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For relative quantification, the formula RQ = 2− ΔΔCt) was used.
However, for a more precise quantification of this formula, further studies including measurements would be desirable to calibrate the formula in detail.
We achieve this by adding relational atoms to the language that represent (implicitly) quantification over all formulas, as in ∀p(Kap→Kbp).
For such languages, which are usually closed under quantification and contain formulas with free variables, Tarski elaborates an appropriate notion of satisfaction, which allows him to introduce the notions of definability, denotation, truth, logical consequence.
Expression values were expressed in relative quantification by applying the formula (1+E -ΔCt where ΔCt = Ctmean target gene - Ctmean reference gene.
The relative quantification of RNA expression was calibrated using formula 2-ΔΔCt method [ 55].
First-order logic formulas involve quantification over component variables.
To determine the relative quantification of gene expression, comparative cycle of threshold (Ct) method with arithmetic formulae (2−ΔΔCt) was used [ 16].
Relative quantification was achieved using the formula 2-ΔΔCt, which relates the amount of cDNA of the specific amplicon to the 18S internal control and the control cDNA.
Relative quantification was assessed using the formula 2-ΔCT.
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