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Marker quantification for each model was carried out over one brain coronal section per mouse at the stereotaxic coordinate showing the wider lesioned area (Figure 1A).
By means of an efficient a posteriori error bound, we can make up for an error quantification for each new parameter value μ in the online stage and thus can make sure that feasibility (and safety/failure) conditions are verified.
To perform that quantification, for each API element mentioned in the threads of our sample, we searched for at least one occurrence in a thread that was considered relevant for documenting it.
For epithelium, we carried out a densitometrical quantification for each component of the ECS.
A five-fold log-scale dilution standard curve was generated for each gene using D. melanogaster genomic DNA in order to perform absolute quantification for each sample.
Protein quantification for each sample was performed by densitometric analysis using KODAK imaging software and normalized to actin in the same sample.
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Mean ± SE from three independent experiments with three quantifications for each determination are shown.
For this, the average % ID/g of the 89Zr and IR quantifications for each sample is plotted - on a logarithmic scale in view of the observed range in % ID/g - against the difference in % ID/g of both measurements for each sample.
First, we compared GLAM predictions and StaRT-PCR quantifications for each sample separately, to assess the performance of the model at the sample level.
In each individual experiment, drug treatments were performed in triplicate and the cAMP and protein quantifications for each sample were performed in duplicate.
Seven concentrations of each plasmid were used with all 24 primer pairs; reactions were run in duplicate wells and in duplicate runs (4 quantifications for each plasmid/primer pair/concentration).
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