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Supporting quantification figure is available as an additional information (Additional file 3).
Figure 2 Proton-Spectrum of a 1 mM solution of aspirin with AurovistTMgold NPs with watergate water suppression;arrows indicate the resonances used for quantification Figure 3 Hydrolysis of aspirin.
The mean slope of the six slopes obtained from a linear regression was considered to be the discrimination power of the algorithm studied for the diffuse heterogeneity quantification (Figure 3C).
The most challenging aspect of data analysis is raw data processing, which involves data conversion, baseline correction, spectrum deconvolution, peak detection and integration, chromatogram alignment, normalization, and compound identification and quantification (Figure 1D).
From the results of chemical quantification (Figure 3), the average proportions of psoralen (82.16%) and bergapten (13.24%) are approximately 20 times of those of luteolin (4.26%) and apigenin (0.34%) in content.
While Mks were differentiating and maturing (Figure 2A), a progressive increase in type I collagen release was observed as revealed by hydroxyproline quantification (Figure 2B).
KLF4 expression was increased 24 and 48 hrs of incubation with PF4 (Figure 2C and quantification Figure 2D; no change at 72 hrs compared to control not shown).
The areas of GSH spectra acquired from human subjects in vivo were applied to the linear concentration curve for quantification (Figure 1D).
Proteins in the individual collected fractions were enzymatically digested and subjected to online LC-MS/MS for protein identification and quantification (Figure 1).
As shown in Figure 4A and the accompanying quantification (Figure 4B), while wt myc-RhoA responds to peroxide similarly to endogenous RhoA with activation over baseline levels, the C16/20A myc-RhoA mutant is resistant to activation by ROS.
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