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After evaluating RNA integrity, complementary DNA was synthesized, followed by the relative quantification each of the genes of interest.
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Both volumes were extracted from the volumes of interests used for the quantification of each type of signal.
There are two kinds of quantification, over each of these sorts.
C Insulin quantification in each of the 12 fractions obtained from the OptiPrep gradient.
The limits of linear quantification of each of the assays were determined with PCR efficiencies of 100±8% and linearities (Pearson's correlation) >0.99.
As this technique uses quantification of each of the targets of interest, it could be more accurate at detecting the subtle bias.
The relative quantification for each of the mRNA levels was calculated using the ΔΔ Ct method as described previously [ 23].
We performed THM water analyses using the "purge and trap" technique for sample concentration and delivery, followed by gas chromatography with mass spectrometric detection (GC/MS) for the separation and quantification of each of the four analytes (U.S. EPA 2003).
The quantification of each of these cell features was performed by counting 3 adjacent high power fields in the top, middle and bottom of the cartilage section using a standardised grid approach.
Quantification of each type of mitochondrial morphology upon p32 knockdown or p32 overexpression was assessed by counting 60 80 cells per condition on three independent occasions.
The primers used for real-time RT-PCR quantification of expression of each gene are described in Table 1.
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