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Quantification details.
Protein identification and quantification details can be found in (Pham et al, 2010; Piersma et al, 2010).
BiFC was measured in the epidermis and considered as affected when the fluorescent signal was equal or lower than 50% of the fluorescent level normally obtained with wild type AbdA (see 'Materials and methods' for quantification details).
The quantification details (methodology, in detail, is in the Experimental Section) are summarized in Table 1. Figure 3 shows the comparative temperature-programmed reaction (TPR) experiments on all the oxides from 500 to 1273 K. On Y2O3, dissociative methane adsorption occurs at temperatures above 1080 K.
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Immunoblots incubated with anti-tubulin (Sigma) were carried out as appropriate for loading control (not shown in the figures for the sake of clarity) and taking into account for band quantification as detailed in the corresponding legends.
Coronal OB sections from developing P3 and adult mice were costained with UEAI, an anti-NCAM antibody, and DAPI to visualize nuclei for glomerular quantification (see for details).
Detail quantification methods are available in SI Materials and Methods.
Accordingly, design, manufacture, and subsequent implementation and insertion of AM materials to meet engineering applications requires detailed quantification of the constitutive (strength and damage) properties of these evolving materials, across the spectrum of metallic AM methods, in comparison/contrast to conventionally-manufactured metals and alloys.
We performed detailed quantification of TRPC4 and 5 in the cortical cell layers and found a uniform distribution of TRPC4 through layers 2 6 with no labeling in layer 1, while TRPC5 mRNA showed dense labeling specifically in layers 2 3 and 5 6.
The experimental procedure and the quantification analysis are detailed in Supplementary Materials and Methods.
This does not allow detailed quantification of the associated risk factors and determinants that are locally important.
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