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Instead, they represent "plausible assumptions and quantification" designed to make executives consider events that may be only remotely possible.
This study presents a real-time reverse-transcription PCR (rt-RT-PCR) assay for hepatitis delta virus (HDV) RNA quantification, designed to clarify the interplay between HDV and hepatitis B virus (HBV) in chronic infection.
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Primers for lncRNA detection and quantification were designed at Universal ProbeLibrary Assay Design Center (http://www.roche-applied-science.com/).com/
Specific primers for quantification were designed using the Universal Probe Library Assay Design Center (Roche, Welwyn Garden City, UK) and target specificity confirmed with Primer-blast (NCBI).
Primers for mRNA quantification were designed using the Universal ProbeLibrary Assay Design Centre (Roche, Welwyn Garden City, UK) and target specificity confirmed with Primer-blast (National Center for Biotechnology Information, Bethesda, MD).
Primer pairs used for quantification were designed using Primer Select software (DNASTAR, Wisconsin, USA) from the available GENBANK and/or ENSEMBL sequences, covering different exons in order to assure the amplification of the cDNA.
The primers used for relative quantification were designed from the coding sequences of BraDof transcription factors in Chinese cabbage; all of the primers used in this experiment are provided in Table 1.
Thanks to our PGD and PND programs, we collected various tissues and cells at various stages of development in embryos and fetuses from m.3243A > G carriers, and investigated local and temporal variation of the mutant load, using a quantification test designed to assess m.3243A > G mutant load at the single-cell level.
The inhibition of blood feeding was assessed by both tick quantification and designing and performing a quantitative PCR (qPCR) to detect the canine hydroxymethylbilane synthase (HMBS) gene in ticks.
The physical data using the non-linear fuzzy set algorithm are applied to quantification of the designed case.
A non-invasive MRI imaging and bone quantification procedure was designed to follow bone regeneration, and these data were extended by μCT imaging and histomorphometry on undecalcified sections for analysis at cellular level.
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