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In addition, FMDV RNA was not detected in several tissue samples (heart, pancreas, spleen, liver, lung and kidney) from C-S8p260 inoculated mice at 48 hours pi by FMDV RNA quantification (data not shown).
We found no difference between hypoxic mice and controls in Cx43 distribution and quantification (data not shown).
We observed moderate colocalization of Pvalb and Drd2 within the ACC via fluorescent double-label in situ hybridization, but levels of Drd2 were too low to allow for accurate quantification (data not shown).
Calibration experiments determined that 25 cycles of 1 min at 94°C, 1 min at 65°C and 2 min at 72°C were optimal for quantification (data not shown).
These longer peptides exhibit a narrower range of ionization efficiencies and are usually the three most intense peptides used for quantification, consequently improving r-protein quantification (data not shown).
After adding dFdC (100 n M) to K8484 cells in vitro, in sharp contrast to the results with K8484 allografts in vivo, it was apparent that dFdCTP was the predominant metabolite rather than GdPC, and in other PDAC cell lines incubated with dFdC the in vitro GdPC concentration was rarely above the lower limit of quantification (data not shown).
Similar(53)
In addition, the hybridization (Fig. 5) and subsequent blot quantifications (data not shown) revealed no apparent increase or decrease in expression of any of the ERAD genes tested among the different strains, suggesting that deletion of a single component of the ERAD pathway does not affect the expression of other components of this pathway.
We confirmed these finding by measuring EB3 movements in several cells and obtaining essentially the same quantification results (data not shown).
We observed that several different synthetic AQUA peptides reporting on the same neurexin isoform often yielded divergent results, making a reliable quantification difficult (data not shown).
The validity of this scoring system was confirmed in the body wall and mid-hindgut, where Likert-quantified scores were comparable to those obtained by counting or by semiautomated quantification respectively (data not shown).
We observed that DNA diluted over 15 times generally gave a quantification cycle (Cq >35 (data not shown); therefore, in the subsequent assays, we used 5× stock DNA dilutions in sterile water.
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