Although there is an experimental evidence of this increase in the sensitivity, its quantification is currently unstudied.
Although this level of PET quantification is currently limited to research studies, efforts are being made to bring kinetic modeling into the clinic to improve the prognostic value of FDG PET still further.
Phage library quantification is currently scored through phage infection of host bacteria, serial dilution, and TU-counting (i.e., individual colony or plaque).
This is the most sensitive MS-based quantification technique currently available with a limit of detection in the low femtomolar range.
With regard to quantification, Pipasic currently relies exclusively on spectral counts.
Further, power Doppler quantification is currently considered a limited tool to assess vascularization, mainly because of the high dependence of gain and other machine settings, attenuation, and reproducibility.
When available, those techniques are helpful for long-term followup and quantification, but currently they are mainly used as a tool for clinical studies [ 57].
This real-time PCR assay allows rapid, sensitive, and specific quantification of all currently defined AdVs into either 2 or 1 multiplex assay for clinical samples.
Absolute quantification is not currently possible for the evaluation of visceral tissues and tumours.
Ongoing research is therefore dedicated to methodological developments that permit correct quantification with the currently available instrumentation for LA-ICP-MS analysis.
