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The Ubiquitin 10 gene was used as an internal quantification control.
5% or 10% of input DNA from both culture conditions were served as positive control for PCR and quantification control.
actin mRNA was amplified using 5'-GACCCAGATCATGTTCGAGACC-3' and 5'-GCATTTGCGGTGAACGATTCCGGG-3' on the same beads to provide a quantification control.
Telomerase activity was calculated according to the following formula: TPG = [(X−X0)/C]∶[(r−r0)/Cr*100], where TPG is the total product generated, X signifies each sample, C represents the 36 bp internal PCR control, r is the TSR8 quantification control.
Gene actin was served as a quantification control.
A heat-inactivated (85°C for 10 min) telomerase control, 1×CHAPS lysis buffer only control, and the TSR8 quantification control were included for each set of TRAP assays according to the manufacturer's instruction.
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In addition to above subarray controls, we also added specific global positive and quantification controls.
After quantification, quality control was assured using the Affymetrix Power Tools Quality Reporter (v1.0.04) quality control metrics.
Some of the particular concerns related with this main problem include the quantification and control of global climate change due to the emissions of greenhouse gases, or the expected decline in global oil production [1].
Future promise is via application of new techniques such as metabolic engineering, accomplishing quantification, and control of metabolic fluxes including inverse metabolic engineering, transcript expression analyses, directed evolution, molecular breeding, and combinatorial biosynthesis.
The diverse nature of environmental risk factors requires an equally diverse interdisciplinary approach to their quantification and control.
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