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Multiplex amplicon quantification consists of a multiplex polymerase chain reaction amplification of fluorescently labelled target and control amplicons, followed by fragment analysis on an ABI3730 DNA Analyser (Applied Biosystems).
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The collected root tissue for Pythium quantification, consisting of six roots, was split in two biological replicates (except at time points 2, 10 & 20 DAG, where the limited material allowed to sample only 1 replicate) and was directly frozen in liquid nitrogen to preserve the DNA.
Cell quantification consisted of using six gridded counting frames that were within the specific subregion of the hippocampus.
The quantification process consists of two steps.
Quantification consisted in using crystal violet as the coloring agent.
An innovative uncertainty quantification framework consisting of; a set-theory based typology, a data library, a classification system, and a corresponding input-output model are put forward to address this research gap as the basis for future work in this field.
However their study was performed using the Inductively Coupled Plasma (ICP) technique, which consists of the quantification of internalised Gd averaged over millions of cells.
Quantification of morphological change typically consists of assessing individual markers at discrete intervals.
The MAQ technique consists of the quantification of fluorescently labelled test and control amplicons, obtained by a single multiplex PCR (mPCR) amplification.
The PUQE, which consists of three quantification items with five answer categories, appears to be a reliable instrument to determine severity of nausea and vomiting during pregnancy [ 2].
The BSAI approach consists of a quantification of the adsorption on the NP surface of different organic probe compounds with diverse structural properties, which senses the different interactions forces between the probe and the NPs surface.
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