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The present work has aimed to provide absolute values for compound quantification by using external standards and analytical curves.
The fragment ions (listed in Table 1) allowed quantification by using one of the three ions as the quantification ion and the other two as qualifiers.
The purpose of this Italian multicenter study was to define pediatric upper reference values for urine particle quantification by using automated flow cytometry.
The fractional cycle number at which the fluorescence passes the fixed threshold (CT values) was used for quantification by using a comparative CT method.
RSV replication and 50% inhibitory concentrations (IC50) were subsequently determined by F protein quantification by using ELISA.
In parallel, 20 replicates were run for background quantification by using only the HIV-1 Gag primer.
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Therefore, all Ct values were transformed into relative quantification data by using the 2-ΔΔCt equation [ 28].
In advance to the evaluation, we established a real-time PCR quantification method by using a LightCycler system.
Results were analyzed with the absolute quantification method by using the second derivative maximum method feature of LightCycler 480 Software v. 1.5.0 SP4 (Roche, Laval, QUE, Canada).
The effects of Tv6 on the autophagy flux was studied by two different methods: LC3II quantification and by using a RFP/GFP LC3 tandem probe that allows the microscopic visualization of autophagosomes and autolysosomes.
cDNA was prepared using GoScript Reverse Transcription System (Promega) prior to APOBEC expression quantification by qPCR using QuantiFast SYBR Green PCR Kit using an ABI ViiA-7 system (Applied Biosystems, Paisley, UK).
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