Sentence examples for quantification by adding from inspiring English sources

In off-gel fractionation, the samples are rehydrated on a gel strip and further separated up to 24 fractions according to pI [ 55]. iTRAQ method can also be improved to perform absolute quantification by adding internal standard peptide.

Termination of reaction for product quantification was achieved by adding 200 µl of a cold solvent mixture (94% acetonitrile + 6% acetic acid, containing tert-butyl phenol as the internal standard) to 500 µl of sample.

The method provides a possibility for quantification of carbohydrates by adding internal standards.

The RTCA SP Station was connected to the RTCA control unit and the xCELLigence system was tested by resistor plate verification prior to the xCELLigence system being placed inside the incubator at a humidified atmosphere at 37°C and 5% CO2. Background quantification was done by adding 100 μl of the appropriate growth medium to the wells.

For all measurements, a quantification of the peaks was achieved by adding a known quantity (between 0.5 and 5.0 μmol) of phenylphosphonic acid as an internal quantification standard before freeze-drying.

Absolute quantification of the product was obtained by adding a known amount of 5-FU to the reaction mixture at the end of the experiment.

We also investigated the effects of inherent PCR-inhibiting compounds in an environmental sample on the quantification using ABC-PCR or real-time PCR by adding the soil extraction solution to PCR mixtures.

The quantification of GSSG was accomplished by derivatizing GSH by adding vinylpyridine to total proteins.

We achieve this by adding relational atoms to the language that represent (implicitly) quantification over all formulas, as in ∀p(Kap→Kbp).

Then, the pH of the supernatant was adjusted to approximately 3.0 by adding 1 M sodium acetate, and the precipitate was used for protein quantification.

Colonies were detected after 10 days of culture by adding 1 mg/ml of 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) reagent and were scored by Image J quantification software (U.S. National Institutes of Health, Bethesda, MD, USA).