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According to this possibilist position, merely possible objects are among the most unrestricted domain of quantification, being constituents of fundamental reality.
The analytical procedure nevertheless resulted in good sensitivity with the LOD (limit of detection) and the LQQ (limit of quantification) being in the lower nanomolar range for both BQ and DMBQ (see Table 2).
Cells were maintained in a cold RPMI-1640 medium (Sigma-Aldrich, USA) for quantification, being dyed with trypan blue and counted in a Neubauer chamber.
Everolimus was measured in whole blood by liquid chromatography mass spectrometry after liquid extraction, its lower limit of quantification being 0.368 ng ml−1.
Paclitaxel was measured in plasma by high-pressure liquid chromatography with ultraviolet detector, lower limit of quantification being 10 ng ml−1.
A variety of methods are used for isolation and quantitation of circulating DNA, with the Qiagen QIAamp Blood Mini Kit (Qiagen, Hilden, Germany) and real-time PCR quantification being most common (van der Vaart and Pretorius, 2010).
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Densitometry quantification was achieved using ImageJ software.
Quantification was performed as in Fig. 1d.
DNA quantification was also carried out.
Quantification was performed by using ImageJ software.
Comparative quantification was normalized to rps26.
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