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In objects whose dimensions exceed the SPECT spatial resolution by several times, quantification based on a calibration procedure similar to the one used in PET seems to be feasible within 10% error limits and even below if a fine-tuning of all acquisition and reconstruction parameters is performed.
Under these conditions, methyl parathion recovery was estimated at approximately 92%, following quantification based on a standard prepared with a 98% methyl parathion pure sample previously obtained by thin-layer chromatography.
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In this study, relative quantification based on the expression of a target gene versus the gyr gene was utilized to determine the transcript level changes between samples.
These experiments demonstrated the feasibility of flow cytometric FRET efficiency quantification based on the use of a single control consisting of the FRET donor expressing cells.
In this report, relative quantification based on the relative expression of a target gene versus a reference gene was utilized to determine transcript level changes [ 57].
However, to our knowledge, there have been no reports on the error structure of reporter ion quantification based on HCD spectra acquired on an LTQ Orbitrap XL ETD mass analyzer, which would be interesting in our view as this was the instrument where a new type of HCD cell with an axial extraction field was first implemented.
The relative quantification ratio was evaluated using quantification based on CT method with an efficiency (E) correction: The expression of GSTs in A375 cells was investigated as previously described (Depeille et al, 2004).
This resulted in (ii) a quantification based on the desired levels of staining intensity (= fraction at different intensity levels).
There are at least two reasons as to why our quantification based on SILAC may potentially have a relatively large variability.
In this report, relative quantification based on the relative expression of a target gene versus GAPDH gene was utilized to determine transcript level changes.
In this report, relative quantification based on the relative expression of a target gene versus the glyceraldehyde-3-phosphate dehydrogenase gene was utilized to determine the transcript level changes.
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