Cells were treated with 50 µM sulindac sulfide following 24-h transfection and then harvested for mRNA quantification at the indicated time points.
Over the last few years, next generation sequencing (NGS) has become a widely adopted technology in many aspects of discovery and translational research, because of its ability to acquire sequence information and quantification at the same time [ 1, 2].
b Below the limit of quantification at these times.
The concentrations measured in the perfusate samples were below the limit of quantification at all time points, indicating limited absorption of the antibiotic from the glandular tissue.
The immunohistochemical quantification at different time points revealed a striking and gradually increasing accumulation of CD68+ cells in the P-407-injected animals compared to the PBS-treated controls.
In our understanding risk is an interdisciplinary concept that acknowledges the benefits and necessary cases for quantification, but at the same time underlines the integration of individual perception and social construction.
This allowed us to include the data from all experiments in an overall quantification, while at the same time accounting for differences in sample sizes, variances, and response levels across different experiments.
For each animal, the cerebellum, hippocampus, and cortex were extracted and stored at −80°C until quantification, at which time 50 mg of each sample was used to conduct the BDNF sandwich enzyme immunoassay.
GFP plot (area in μm) of spheroid growth delay after (A) 5-FU, Cycloheximide, Etoposide, Actinomycin D, (B) Staurosporine, 6-TG, and Hydrocortisone (10 μM) treatment and quantification at indicated time points (data are averages ± SD, n = 3).
