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13 Standard complete two-dimensional echocardiography was performed in all individuals, with chamber quantification assessed according to published criteria.
The histological quantification assessed the total area of myelin in relation to the cross-sectional area of the sciatic nerve by quantifying pixels in digital images that were processed and transformed into a binary display (black and white).
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This paper highlights the economic contributions of natural resources to rural livelihoods and represents one of the first comprehensive income quantifications assessing household- and landscape-level determinants of resource use in the Amazon.
Protein quantification was assessed using the Bradford protein assay44.
DPA quantification was assessed using HPLC, and samples were taken both immediately and 2 h after the pressure treatment.
For this reason, lipid quantification was assessed by using an average of extinction coefficients for all fatty acids (Figure 3): y = 1.327 × 10- 2 x + 2.911 × 10- 2 (R2 = 0.999), where y is the absorbance measured at 440 nm and x is the fatty acid concentration in milligrams per liter.
The number of VOCs identified, their chemical classes and the repeatability of their quantification were assessed when 4 ml urine samples from healthy volunteers were analysed fresh, frozen at −80 °C, freeze-dried, with the addition of 1 ml of saturated NaCl solution (salt), with the addition of 1 ml of 5M HCl solution (acid) or with the addition of 1 ml of 5M NaOH solution (base).
The accuracy of the basic real-time LCR protocol for mutant DNA quantification was assessed by testing the genomic DNA pools prepared from extracted samples containing the V136 polymorphism at different pre-determined frequencies (50%, 25%, 6.25%, 1.56%, and 0.39%).
Invasion quantification was assessed as previously described.
RNA quantification was assessed by spectrophotometry (NanoDrop Technologies).
mRNA quantification was assessed using the 2−ΔΔCt method.
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