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For GFP intensity quantification, animals were photographed under a GFP filter and the average brightness was determined for each photograph by Lucia Nikon software.
For quantification, animals carrying the extrachromosomal arrays were placed into a COPAS Biosort (Union Biometrica) and animals expressing dsRed were separated from non-transgenic animals in the gating window.
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For glycogen quantification experiments, animals were returned to room air and immediately stained with iodine.
MRI is proving to be a very useful tool for sodium quantification in animal models of stroke, ischemia, and cancer.
His own research was on the canarypox virus, which he used in developing a chick embryo assay for the isolation and quantification of animal viruses.
Although quantification of animal traits exists for the species studied here (Herrera 1984; Jordano 1987), our system is unfortunately too small (36 interactions) to support models with these additional explanatory variables.
No significant influence from different angulations of the ROIs could be found (data not shown), which indicates that fine-tuning of the ROI position is not a prerequisite for accurate BLI quantification in animal models of subcutaneously implanted catheters.
The most widely used assay employs HPLC on a reversed phase C18 column [ 1, 16– 19], originally developed in 1981 by Faris et al. [ 17] for desmosines quantification in animal tissues.
Important variables that could be used to improve standardization of CHI models include the reproducibility and intensity of mechanical inputs, construction of the CHI device, location and direction of impact, and quantification of animal head kinematics and injury outcomes (Fig. 4).
For immunohistochemistry and immunofluorescence analysis, values represent the means±s.e.m. of at least five quantifications per animal.
Therefore, evaluation of the input function without blood sampling would be desirable for CBF quantification in small animals.
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