The effects of each polymer fiber on cell adhesivity, proliferation, morphological changes, and synthesis of the extracellular matrix were analyzed by quantitative a cell attachment test, DNA quantification, light and scanning electron microscopy, semi-quantitative RT-PCR, and immunohistochemical analysis.
Image quantification and densitometric scanning of immunoblots were done using Image J software (Bethesda, MD, USA).
At the end of the DC process, biopsies were taken from decellularized veins (DV) and processed histologically with Hematoxylin Eosin (HE), Masson's Trichrome (MT) and Verhoeff Van Gieson (VVG) staining, DNA quantification, Luminex®, scanning and transmission electron microscopic analysis, tensile strength and extracellular matrix (ECM) quantifications.
This study was to establish a method for simultaneous separation of three monoterpenes (linalool, cineole and β-caryophyllene) and two coumarins (scopoletin and scoparone) on a single TLC plate by using double development because of their obvious difference of polarity [11, 12], and quantification by scanning densitometry.
Product separation by SDS-PAGE, visualization by fluorescent scanning, quantification, and normalization within each lane were performed as described for the substrate selection experiments.
To bridge this gap, we established methods for both imaging and automated analysis that encompasses staining, scanning, reconstruction, quantification and modelling (Fig. 1).
As the amount of truncation may vary across scans and subjects, it will be difficult to ensure proper quantification across scans and subjects.
Further details about methodology of peptide administration, plasma hormone quantification, fMRI scanning, picture processing task, and analysis can be found in Supplemental Experimental Procedures.
Saturation effects are generally only a problem for survey scan-based quantification and are rarely encountered for tandem MS-based methods.
Analysis was carried out via mechanical tensile testing, DNA quantification, scanning electron and light microscopy, and a metabolic assay, which was used to give an indication of the biocompatibility of each decellularization method.
