Sentence examples for quantification and reference from inspiring English sources
Some hold that some plural referring expressions are irreducible to singular predication, quantification, and reference (Boolos 1984; McKay 2005; Yi 2005).
Sentences such as "The cheerleaders formed a human pyramid," or "Those dogs are sick" are examples of plural predication, quantification, and reference.
Alternatively, as sequencing-based technologies provide digital quantitative output, absolute quantification ensues, and reference gene identification becomes more accurate.
Additional file 3: Quantification of cytokine and reference gene expression in unstimulated/stimulated PBMC assays.
All analyses were performed using the quantification of cytokine and reference genes as number of copies.
The quantification of target and reference (GAPDH) genes was performed in triplicate on a LightCycler® 480 II (Roche, Applied Science) using a SYBR green-based assay (BioRad, USA).
Finally, we checked the stability of candidate reference gene expression, performed absolute quantification of cytokine and reference gene mRNA in whole blood samples and relative expression of cytokine mRNA in stimulated PBMC samples.
Quantification of cytokine and reference genes in unstimulated and ConcanavalinA-stimulated PBMC samples are presented in Additional file 3. First, the optimal number of reference genes required for relative expression was determined using the geNorm application.
Quantification of cytokine and reference gene expression was checked by amplification of cDNA synthesized from ConcanavalinA-stimulated and unstimulated PBMC total RNA from three independent experiments for each species.
The relative expression ratio (Er) of cytokine genes in ConcanavalinA-stimulated cells (n=3) compared to unstimulated cells (n=3) was calculated using the quantification of cytokine and reference gene as number of copies.
Hence genome size, ploidy and unknown sources of contaminating DNA could affect amplification performance by altering the ratio of target to non-target DNA presence and hence potentially making target quantification and comparisons with reference samples and standards inaccurate.
