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Quantification and purification of all cDNA samples were assessed by using NanoDrops spectrophotometer (Thermo Scientific).
RNA quantification and purification was determined by measuring absorbance at 260 and 280 nm.
Evaluation of RNA quantification and purification was carried out by measuring absorbance at 260 and 280 nm.
Hexane layers were pooled, dried under nitrogen, resuspended in 100 μL methanol, frozen at −80°C for ∼5 min, centrifuged briefly, and injected onto HPLC system 1 for quantification and purification.
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Part III looks at the purification, quantification, and analysis of the ECM proteins, and Part IV describes computer-assisted methods such as quantifying fibrillar collagen alignment and exploring the nano-surface of collagen with atomic force microscopy (AFM).
RNA isolation, purification, quantification and cDNA synthesis are described elsewhere [ 48].
After purification, quantification, and validation, the validated DNA libraries were sequenced on an Illumina MiSeq Sequencing System following the manufacturer's standard workflow.
Recombinant production of MTs helps to circumvent some of the problems associated with direct isolation, and expression as a GST fusion offers simple possibilities for purification, quantification, and detection [ 30].
Higher throughput than reported here (>92 samples typed at a time) is possible for the entire pre-emPCR process in a lab setting utilizing liquid handling robotics for RNA extraction, cDNA synthesis, PCR amplification, SPRI purification, quantification, and pooling, similar to the methods employed by Erlich et al. [ 23].
After adding the sample barcodes to the fully documenting MS Excel run protocol, formatted work lists for PCR setup, purification, quantification and pooling as well as a MID-sample association file for ATF software were automatically generated by Excel Macros (VBA).
Extraction and purification of samples and quantification of phytohormones was performed following a procedure described in Chiwocha et al. [ 89, 90].
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