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10.7554/eLife.07861.009 Figure 4. Terrein quantification and expression of terrein cluster genes in nitrogen regulator mutants.
Protein quantification and expression analysis of IL-1 β and TNF- α were performed using an enzyme-linked immunosorbent assay (ELISA) method that was specific for each cytokine.
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The same idea can be easily adapted to the three stages of RNAseq analysis as well: raw data, expression quantification, and differential expression analysis.
Protein quantification and differential expression of proteins were done using a proprietary software licensed from Eli Lilly and Company [32].
We then used a combination of genetic mapping, fast neutron mutant analysis, allelic diversity, transcript quantification and transient expression complementation studies to confirm the identity of the candidates.
Some of the animals (n = 5 7, per group) were used for DNA quantification and gene expression analysis and the others (n = 5, per group) to perform morphometric and immunohistochemical analysis in WAT.
Mice were sacrificed and pulmonary tissue was collected for histopathology, collagen quantification, and gene expression analysis.
Thereafter, mice were killed by decapitation, blood was immediately collected, and all the tissues were dissected for mercury quantification and gene expression analysis.
Recent advances in real-time PCR analysis have led to significant improvements in the accuracy and simplicity of DNA quantification and gene expression analysis.
Based on GAG quantification and gene expression analysis, encapsulated passage 1 cells cultured in growth medium displayed the best ectopic chondrogenesis.
Gas production was recorded at 2-hour intervals and at the end of the incubation the liquid layer of each syringe was sampled for pH, volatile fatty acids analysis, bacterial quantification, and gene expression.
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