S-nitrosocysteine (CSNO) was synthesized as described elsewhere [ 18] by incubation of L-cysteine with acidified sodium nitrite and quantification by absorbance at 334 nm using a molar absorption coefficient of 0.74/mM/cm.
The biofilm quantification was performed by Microplate Reader (Molecular Devices), and absorbance at a wavelength of 570 nm (A570) was reported.
When considering phenol chloroform based extraction methods (BP1 and BP2 methods) the DNA quantification and purity determination using UV absorbance at 260 nm may be error prone due to the high extinction coefficient of phenol at 260 nm resulting in lower detectable yields (Yang 2008).
The supernatant was used for protein quantification with the Bradford assay method (Bio-Rad) and absorbance at 595nm was measured using PowerWave XS spectrophotometer (Witec AG).
For photometric quantification, colonies were destained with 2% SDS and absorbance was measured at 562 nm using a SynergyHT plate reader and Gen5 software (BioTek, Winooski, VT, USA).
Also, the deviations were higher for deeper tissues, pointing to a significant scattering and absorbance of the NIR light, limiting the quantification of tissue uptakes by fluorescence imaging and necessitating the quantification of organ uptakes by PET.
For quantification of soluble film loading, films were dissolved in PBS and absorbance values converted to concentration via a standard curve made in PBS.
Quantification of malvidin 3-glucoside was performed by external calibration and absorbance measurement at 530 nm using the TLC Scanner 4 (CAMAG, Muttenz, Switzerland).
The identification and quantification of CPT was performed based on the retention time and absorbance spectra of CPT reference solutions (0.1, 0.05, 0.01, 0.05, 0.001 mg ml-1) (Sigma, St . Louis MO, USA).
For spectrophotometric quantification, the biofilm was dissolved in 4 ml of 75%% ethanol and absorbance noted using a spectrophotometer at 570 nm.
