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Expression levels of genes of interest were calculated relative to ACTIN using the comparative quantification analysis method (Rotogene-5; Corbett Research), which takes into account the amplification efficiency of each primer set.
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Expression was normalized relative to the control (Actin) using Roche LightCycler advanced relative quantification analysis (E-method, http://www.roche-applied-science.com/shop/products/gene-quantification-on-the-lightcycler-480-system) and fold changes (relatively to B1 or R1 stage) are shown in Figure 6.
Limit of detection and limit of quantification of the analysis method were <18.0 and <53.0 ng/ml for β-IO, CC, and TMT, whereas 156 and 474 ng/ml were determined for DHA, respectively.
We approached the problem of localizing electroreception in rabbits by using recurrence quantification analysis (RQA), a novel method of nonlinear analysis designed to detect small deterministic changes in a larger signal irrespective of considerations involving stationarity.
Data elaboration was performed as relative quantification analysis using the ΔΔCt method.
The method for quantification analysis of the images was the same as reported previously [27].
These experiments were designed to develop a rapid, repeatable and accurate analysis method for the quantification of galacturonic acid of pectins.
We set out to (i) review the different methods used and (ii) use simulations to explore how the data analysis method influences the quantification of wetland hydraulics and pollutant removal.
For quantification analysis the comparative threshold cycle (Ct) method was used.
The expression level of each gene was measured via RNA-Seq quantification analysis using the RPKM [ 38] method (reads per kb per million reads).
Immunoblot quantification was done with the gel analysis method in ImageJ 1.47 m.
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