Sentence examples for quantification amounts from inspiring English sources

Therefore, the reported figures cannot be considered as absolute expression levels of the genes of interest, and can only be used as relative quantification amounts.

After protein quantification, equal amounts of protein (50 μg/lane) were separated by 10 or 15% polyacrylamide gel electrophoresis (PAGE) and transferred to Immobilon-P transfer membranes (Millipore, Billerica, MA, USA), which were then blocked and exposed to antibodies.

The standard curve of human genomic DNA consistently yielded R2 (goodness-of-fit) values > 0.98 for the human 18S rRNA gene qPCR, which enabled quantification of amounts of cellular material in BAL fluid.

For quantification, the amount of miR was normalized to the amount of U6 miR using the 2 − DDCT method.

For correct quantification, the amount of cDNA used for the amplification of GAPDH was adjusted according to the amount of each of the amplified genes.

Based on band quantification, the amount of Tim50 extracted by SMA at the concentration used was nearly equivalent (within 5%) to the amount extracted by DDM.

The number of cells on the scaffold was determined by the fluorometric quantification of amount of cellular DNA.

After the incubation period, the cells were washed and whole cell lysates for comparative quantification (the amount of proteins in unstimulated cells were set to 1) of total protein concentration prepared.

For a reliable mass-spectrometric quantification, the amount of tissue obtained from our experimental setup is limiting.

100 bp ladder was also loaded as standard for quantification of amount and confirmation of PCR product size.

The quantification of amount of dsDNA and unwound ssDNA and dsRNA and unwound RNA was done as described in experimental procedures section.