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For fluorescence quantification, acquisition time was 4 msec for the VFs and 100 msec for the nuclei.
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Laser power was set at 6% with a constant gain setting of 780, for all quantification acquisitions.
Quantification and acquisition of array images were done using Affymetrix Gene Chip Operating Software (GCOS) version 1.4.
For the quantification, the acquisition was averaged and expressed as the ipsi-to-contralateral hind limb ratio of arbitrary units.
VS contributed in the collection of tumor material, quantification and acquisition of data and writing of the manuscript.
The images were acquired within 30 min. For fluorescent quantification, image acquisition settings were below saturation at a resolution of 1024 × 1024 pixels (12 bit).
EdK participated in the design of the study, the collection of tumor material, the construction of the tissue micro array, the immunohistochemical stainings, quantification and acquisition of data, performed the statistical analysis and participated in writing of the manuscript.
For quantification of immunoreactivity, acquisition of images was performed using the DotSlide Virtual Slide System (Olympus, Tokyo, Japan).
Image acquisition, quantification and data analysis were performed using the Affymetrix GeneChip Operating Software (GCOS) v1.4.0.036.
MAS, version 5.0, [ 10] was used for data acquisition, quantification, and normalization.
Quantification using an acquisition interval of 15 s show that 89% ± 6% of filopodia emerge from actin patches (24 patches form 10 neurons; 3 independent experiments).
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