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The concentrations of interleukin-6 and -8 (IL-6 and IL-8) were determined using enzyme-linked immunosorbent assay (ELISA) quantification according to manufacturer's recommendations.
Motivated by aforementioned facts, in this paper, a new method, which is suitable for clustering and identifying the unusual electricity consumptions and their quantification according to the nature of irregularity, is proposed.
The developed methods are validated with respect to linearity, accuracy and precision, specificity, limit of detection, and quantification according to the guidelines set by International Conference on Harmonization (ICH Q2 R1 2005).
Illumina libraries were clustered for sequencing immediately following sample quantification according to manufacturers recomendations (Illumina).
ELISA kits were purchased from BD Biosciences (TNF, IL-6, IL-10, MCP-1) and R&D Systems (MIP-1α, GRO-α), and were used for cytokine/chemokine quantification according to the manufacturer's recommendations.
β-Actin served as endogenous control for relative quantification according to the ΔΔ Ct-method [ 18, 21].
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For all antibodies, titrations were performed and specificity was assessed using isotype control antibodies before imaging and quantifications, according to standard procedures from the Yale University Center for Cell and Molecular Imaging.
Color quantification was according to the CIE lab system (Commision Internatinale de l'Eclairage, L*, a*, b*) presented in Table 1.
Hence, quantification theory, according to Peirce, is not a matter of an extension of formal vocabulary, but an expansion into territory that is qualitatively different from what Aristotelian logic covers.
Superoxide radical quantification was according to the method of Myouga et al [59].
NAD+-NADH measurements were done using BioVision (Mountain View, CA) NAD+/NADH Quantification kit according to the manufactures instructions.
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