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Again, marking was very low in this study and vector DNA did not reach the quantifiable range of the assay (0.38% of cells analyzed) in any blood cell sample at any time point.
This assay was developed for the larger quantifiable range of NGAL compared with the existing competitive assay.
Blood concentrations used in toxicology studies may be much higher and, therefore, will need to be diluted accordingly to bring the sample signal into the quantifiable range of the assay.
Numbers vary between cytokines and growth factors because of slight variations in the number of values that were excluded from each group (those values that were out of quantifiable range of the assay) We then performed limited multivariable modeling to evaluate the adjusted association between PlGF and sFlt-1 levels and scleroderma-associated PH.
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In each assay, the target amplification from the RNA sample was out of the quantifiable range.
Plasma HIV-1 RNA concentrations were analysed using the log10 scale; values above or below the quantifiable range were assigned values at the respective limits of the assay.
The manufacturer reported that precision (coefficient of variation) is 12.1% for BLLs in the lower quantifiable range (average BLL 5.3 μg/dL), 7.6% and 5.5% for BLLs in the intermediate quantifiable range (averages 11.0 and 22.9 μg/dL, resp ., and 3.5% for higher quantifiable BLLs (average 51.7 μg/dL) [ 24].
(1) MFI values outside the quantifiable range.
fabp1b.2 mRNA was detectable in intestine and brain of fish fed the four diets, but the levels in liver and muscle were below the quantifiable range in RT-qPCR assays (data not shown).
Quantifiable ranges were determined using these criteria for both 2- and 18-h reactions.
Six cytokines were detected using CBA Th1/Th2 cytokine kit, Th1/Th2 cytokine standard curves were shown in Figure 2. Cytokine concentration of plasma in patients was calculated according to the standard curves with 20 pg/ml ~ 5000 pg/ml quantifiable ranges.
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