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Again, marking was very low in this study and vector DNA did not reach the quantifiable range of the assay (0.38% of cells analyzed) in any blood cell sample at any time point.
(1) MFI values outside the quantifiable range.
In each assay, the target amplification from the RNA sample was out of the quantifiable range.
This assay was developed for the larger quantifiable range of NGAL compared with the existing competitive assay.
All samples in the standard curves and experimental runs were run in triplicate and the Cq values fell within the linear quantifiable range for the relevant standard curve.
Plasma HIV-1 RNA concentrations were analysed using the log10 scale; values above or below the quantifiable range were assigned values at the respective limits of the assay.
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Quantifiable ranges were determined using these criteria for both 2- and 18-h reactions.
Accuracy within the defined quantifiable ranges was high (0.1 13 % error) under all conditions in plasma, including those with other toxins expected to be present during infection.
This new method does not require patient-specific reagents because it utilizes standard consensus primers and also has standardized sensitivity and quantifiable ranges.
Six cytokines were detected using CBA Th1/Th2 cytokine kit, Th1/Th2 cytokine standard curves were shown in Figure 2. Cytokine concentration of plasma in patients was calculated according to the standard curves with 20 pg/ml ~ 5000 pg/ml quantifiable ranges.
Quantifiable concentrations ranged from 0.17 to 54.4 ng/g (bee body weight) for individual pesticides.
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